Blood Fractionation Chromatography

Fractionation is important to understand someone’s health. How we can simulate blood fractionation through a simple, inexpensive ink spread experiment more about blood fractionation and liquid chromatography. Cotton will spread on ink the most because it has the most pores. Pilot ink spreads fast because it is water-based (low viscosity). Higher temperatures should result in more spread because of lower viscosity. We measured the ink spread by taking the average between high and low ink mark points. We check our measurement repeatability and determined our sample size. The results repeatedly showed that nylon was the material with the least ink spread, with water not spreading at all, pilot ink barely spreading, and India ink spreading very little. For polyester, water spread much better than nylon. It was clearly shown that higher temperatures meant more spread. We heated the cotton cloth only since using heat transfer from the water to the ink is too long and the water vapor would block the pores from absorbing the ink. We learned more about using statistics graphs and tables. We also learned how to control the experiment and determine the sample size through teamwork building. We would thank our teachers and parents for advising.